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Journal
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Immunology and Infection
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总蛋白提取和二维凝胶电泳方法<em>鼻疽</em>物种
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Total Protein Extraction and 2-D Gel Electrophoresis Methods for
Burkholderia
Species
Please note that all translations are automatically generated.
Click here for the English version.
总蛋白提取和二维凝胶电泳方法<em>鼻疽</em>物种
DOI:
10.3791/50730-v
•
08:31 min
•
October 15, 2013
•
Billie Velapatiño
,
James E. A. Zlosnik
,
Trevor J. Hird
,
David P. Speert
1
Department of Pediatrics, Centre for Understanding and Preventing Infection in Children
,
University of British Columbia
Chapters
00:05
Title
01:08
Protein Extraction
02:41
First-dimensional Isoelectric Focusing
04:06
Second-dimension SDS Polyacrylamide Gel Electrophoresis and Sliver Staining
07:14
Representative Protein Extraction Results
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在
洋葱伯克霍尔德
属的成员是重要的临床意义病原体。我们描述了一种菌体总蛋白的提取,采用机械破碎,和2-D凝胶电泳随后蛋白质组学分析。
Tags
Protein Extraction
Burkholderia Species
2-D Gel Electrophoresis
Proteomics
Isoelectric Focusing
SDS-PAGE
Silver Staining
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