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总蛋白提取和二维凝胶电泳方法<em>鼻疽</em>物种
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
DOI:

08:31 min

October 15, 2013

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Chapters

  • 00:05Title
  • 01:08Protein Extraction
  • 02:41First-dimensional Isoelectric Focusing
  • 04:06Second-dimension SDS Polyacrylamide Gel Electrophoresis and Sliver Staining
  • 07:14Representative Protein Extraction Results
  • 07:58Conclusion

Summary

Automatic Translation

洋葱伯克霍尔德属的成员是重要的临床意义病原体。我们描述了一种菌体总蛋白的提取,采用机械破碎,和2-D凝胶电泳随后蛋白质组学分析。

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