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Bioengineering
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编程干细胞的治疗性血管新生使用可生物降解高分子纳米微球
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Bioengineering
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JoVE Journal
Bioengineering
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
Please note that all translations are automatically generated.
Click here for the English version.
编程干细胞的治疗性血管新生使用可生物降解高分子纳米微球
DOI:
10.3791/50736-v
•
09:01 min
•
September 27, 2013
•
Michael Keeney*
1
,
Lorenzo Deveza*
1,2
,
Fan Yang
2
1
Department of Orthopaedic Surgery
,
Stanford University
,
2
Department of Bioengineering
,
Stanford University
Chapters
00:05
Title
01:58
Polymer Synthesis
03:57
Nanoparticle Preparation and Transfection
04:55
Cell Injections
05:49
Bioluminescence Imaging
06:38
Tissue Harvest Procedure
07:07
Results: Angiogenesis after Nanoparticle Transfection
08:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了编程干细胞的方法,使用可生物降解的聚合物纳米颗粒以过表达的治疗因子的血管生成。所述工艺包括聚合物的合成,转染脂肪来源的干细胞
在体外
和验证编程的干细胞的功效,以促进血管生成的小鼠后肢缺血模型。
Tags
Stem Cells
Angiogenesis
Therapeutic Angiogenesis
Biodegradable Polymeric Nanoparticles
Vascular Growth
Ischemic Diseases
Gene Therapy
Adipose-derived Stem Cells
VEGF
Hindlimb Ischemia Model
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