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Journal / Biology / Protokoll för Implementera en
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Biology

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JoVE Journal Biology
Protokoll för Implementera en
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Protokoll för Implementera en

Article DOI: 10.3791/50762 • 16:11 min • September 16th, 2013
September 16th, 2013 •
*1, *2, 3, 4, 2, 4
1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota
* These authors contributed equally

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Denna femdagars-protokollet beskriver alla steg, utrustning och extra programvara som krävs för att skapa och driva en effektiv endogen Escherichia coli baserad TX-TL cellfria expressionssystem från grunden. Med reagenser, tar det protokoll 8 timmar eller mindre att installera en reaktion, samla in och bearbeta data.

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Cellular Biology bioteknik Syntetisk biologi kemi Techniques Syntetisk molekylärbiologi reglerteknik TX-TL cell-yttrandefrihet in vitro transkription-translation cellfria proteinsyntes syntetisk biologi systembiologi Escherichia coli-cellextrakt biologiska kretsar biomolekylär kopplingsdäck
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