/
/
Systematische Analyse von<em> In-vitro-</em> Zellroll Mit einer Multi-Well-Platte mikrofluidischen System
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Division of Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital, 2Center for Regenerative Therapeutics,Brigham and Women’s Hospital, 3Harvard Medical School,Harvard University, 4Harvard Stem Cell Institute,Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering,Massachusetts Institute of Technology
Chapters
- 00:05Title
- 01:24Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin
- 02:07Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel
- 03:22LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin
- 04:09HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels
- 05:41Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System
- 10:22Conclusion
Diese Studie verwendet eine Multi-Well-Platte mikrofluidischen System, deutlichen Erhöhung des Durchzellroll Studien unter physiologisch relevanten Scherströmung. Angesichts der Bedeutung der Zellvoll in der Mehrschrittzellen Homing Kaskade und die Bedeutung der Referenzzelle nach systemischer Abgabe exogener Populationen von Zellen in Patienten, bietet dieses System als Screening-Plattform zellbasierte Therapie verbessern Potential.
