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Análise sistemática de<em> In Vitro</em> Célula de rolamento usando um sistema microfluídicos Multi-bem placa
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1Division of Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital, 2Center for Regenerative Therapeutics,Brigham and Women’s Hospital, 3Harvard Medical School,Harvard University, 4Harvard Stem Cell Institute,Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering,Massachusetts Institute of Technology
Chapters
- 00:05Title
- 01:24Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin
- 02:07Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel
- 03:22LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin
- 04:09HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels
- 05:41Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System
- 10:22Conclusion
Este estudo utilizou um sistema de microfluídica multi-bem placa, aumentando significativamente o rendimento dos estudos rolantes célula sob fluxo de cisalhamento fisiologicamente relevante. Dada a importância da laminagem celular na célula de multi-passo homing cascata e a importância de homing celular após entrega sistémica de populações exógenas de células em pacientes, este sistema oferece a possibilidade de uma plataforma de pesquisa para melhorar a terapia de base celular.
