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Analisi sistematica delle<em> In Vitro</em> Cella rotolamento Utilizzando un piatto multi-ben Sistema Microfluidic
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1Division of Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital, 2Center for Regenerative Therapeutics,Brigham and Women’s Hospital, 3Harvard Medical School,Harvard University, 4Harvard Stem Cell Institute,Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering,Massachusetts Institute of Technology
Chapters
- 00:05Title
- 01:24Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin
- 02:07Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel
- 03:22LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin
- 04:09HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels
- 05:41Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System
- 10:22Conclusion
Questo studio ha utilizzato un sistema di microfluidica piastra multi-pozzetto, aumentando notevolmente la velocità di rotolamento studi cella in flusso di taglio fisiologicamente rilevanti. Data l'importanza della laminazione di cellule nella cella multi-step homing a cascata e l'importanza di homing delle cellule dopo la consegna sistematica delle popolazioni esogeni di cellule nei pazienti, questo sistema offre un potenziale come piattaforma di screening per migliorare la terapia cellulare.
