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Mouse Genome Engineering Bruke Designer nukleaser
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JoVE Journal Biology
Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Bruke Designer nukleaser

DOI:
10.3791/50930-v

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12:04 min

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April 02, 2014

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, , ,
1Institute of Laboratory Animal Science,University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering,University of Minnesota

Chapters

  • 00:05Title
  • 01:18Identifying TALEN Target Sites
  • 02:30Embryo Isolation
  • 04:23Embryo Microinjection
  • 06:33Embryo Transfer
  • 09:15Identifying Founders with Targeted Mutations
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

Designer nukleaser som sink finger nukleaser (ZFNs) og transkripsjons aktivator lignende effektor nukleaser (Talens) kan brukes til å endre genomet muse preimplantation embryoer ved å utløse både ikkehomologe ende bli (NHEJ) og homolog rekombinasjon (HR) trasé. Disse fremskrittene muliggjøre rask generasjon av mus med presise genetiske modifikasjoner.

Tags

Mouse Genome EngineeringDesigner NucleasesZinc Finger Nucleases (ZFNs)Transcription Activator-like Effector Nucleases (TALENs)CRISPR/Cas9Site-specific Genome ModificationKnockoutKnock-inTransgenic MiceGenome EditingMicroinjectionFertilized Mouse Oocytes

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