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JoVE Journal
Immunology and Infection

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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
 
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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Article doi: 10.3791/51033
August 23rd, 2013

Summary August 23rd, 2013

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Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.

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