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Fjernelse af en enkelt celle fra otte-celle embryoner af amphipoden Crustacean<em> Parhyale hawaiensis</em
JoVE Journal
Biology
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JoVE Journal Biology
Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
DOI:

10:55 min

March 16, 2014

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Chapters

  • 00:00Title
  • 00:14Introduction
  • 01:07Introduction: Animal Culture
  • 03:11Procedure: Removing a Single Cell from the 8 Cell Stage during Parhyale Development
  • 04:37Gathering Embryos: Materials
  • 06:14Ablating g: Materials
  • 07:07Ablating g: Procedure
  • 10:18Representative Results

Summary

Automatic Translation

Amphipoden Parhyale hawaiensis er en lovende model organisme til studier af krebsdyr embryologi og sammenlignende leddyr udvikling og evolution. Denne protokol beskriver en metode til manuel fjernelse af enkelte blastomeres fra tidlig spaltning stadie embryoner fra Parhyale.

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