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Identification des modifications post-traductionnelles des protéines végétales Complexes
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Identification des modifications post-traductionnelles des protéines végétales Complexes

Article DOI: 10.3791/51095-v • 10:07 min • February 22nd, 2014
February 22nd, 2014 •
1, 2, 2, 1,2, 3, 1
1School of Life Sciences, University of Warwick, 2The Sainsbury Laboratory, Norwich Research Park, 3Research School of Biology, The Australian National University

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Nous décrivons ici un protocole pour la purification et la caractérisation de complexes de protéines végétales. Nous démontrons que par immunoprécipitation une seule protéine dans un complexe, afin que nous puissions identifier ses modifications post-traductionnelles et ses partenaires d'interaction.

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Biologie végétale Numéro 84 interactions plantes-microorganismes la purification de protéine complexe la spectrométrie de masse la phosphorylation des protéines Prf Pto AvrPto AvrPtoB
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