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Biology
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植物蛋白复合体的翻译后修饰的鉴定
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Identification of Post-translational Modifications of Plant Protein Complexes
Please note that all translations are automatically generated.
Click here for the English version.
植物蛋白复合体的翻译后修饰的鉴定
DOI:
10.3791/51095-v
•
10:07 min
•
February 22, 2014
•
Sophie J. M. Piquerez
,
Alexi L. Balmuth
,
Jan Sklenář
,
Alexandra M.E. Jones
2
,
John P. Rathjen
,
Vardis Ntoukakis
1
School of Life Sciences
,
University of Warwick
,
2
The Sainsbury Laboratory
,
Norwich Research Park
,
3
Research School of Biology
,
The Australian National University
Chapters
00:05
Title
01:37
N. benthamiana
and
A. thaliana
Protein Samples
03:24
Protein Immunoprecipitation
05:32
Protein Digestion
07:48
Results: Identification of Post-translational Modifications of Tagged Proteins
09:13
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述的一个协议,用于植物蛋白质复合物的纯化和表征。我们证明了在一个复杂的免疫沉淀一个单一的蛋白质,这样我们就可以识别其翻译后修饰及其相互作用的合作伙伴。
Tags
Plant Proteins
Post-translational Modifications
PTMs
Protein Complexes
Immune Complexes
NB-LRR Proteins
Mass Spectrometry
Protein Purification
Immunoprecipitation
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