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电动细胞基质阻抗传感的内皮细胞增殖，屏障功能的定量和能动性

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Please note that all translations are automatically generated. Click here for the English version.

电动细胞基质阻抗传感的内皮细胞增殖，屏障功能的定量和能动性

DOI:

10.3791/51300-v

•

12:30 min

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March 28, 2014

•

Robert Szulcek, Harm Jan Bogaard, Geerten P. van Nieuw Amerongen

¹Department of Pulmonary Diseases,Institute for Cardiovascular Research, VU University Medical Center, ²Department of Physiology,Institute for Cardiovascular Research, VU University Medical Center

Chapters

00:05Title
01:23Preparing the Arrays
04:19Initiating Data Collection
06:17Manipulating the Cells During Data Collection
07:52Data Analysis and Modeling of Rb and Alpha
08:40Typical Experiment and Analysis
11:55Conclusion

Summary

Automatic Translation

此协议的评论电器细胞 - 基底阻抗传感方法来记录和分析的贴壁细胞的阻抗谱细胞附着，增殖，运动，和细胞反应的药理学和毒性刺激的量化。检测血管内皮细胞通透性和细胞 - 细胞和细胞 - 基质接触的评估被强调。

Tags

Electric Cell-substrate Impedance Sensing ECIS Impedance-based Measurement Cell Behavior Quantification Cell Attachment Cell Spreading Cell Proliferation Barrier Function Cell Motility Wound Healing Endothelial Cells

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