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高通量MHC II类分子结合分析抗原表位肽的定量分析
JoVE Journal
Biology
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JoVE Journal Biology
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
DOI:

07:59 min

March 25, 2014

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Chapters

  • 00:05Title
  • 01:20Binding and Capture (Day 1)
  • 03:19Detection and Analysis (Days 2-3)
  • 05:06Results: Binding Curves for Representative Test Peptides
  • 07:29Conclusion

Summary

Automatic Translation

生物化学测定重组人MHC II类分子可提供快速,定量分析上市免疫原性表位的识别,缺失或设计。这里,缩放到384孔板的肽-MHC II类分子结合测定法进行说明。这种成本效益的格式应该证明在蛋白质去免疫和疫苗的设计和开发领域非常有用。

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