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Biology
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细胞标记和注射胚胎发展小鼠心脏
JoVE Journal
Biology
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JoVE Journal
Biology
Cell Labeling and Injection in Developing Embryonic Mouse Hearts
Please note that all translations are automatically generated.
Click here for the English version.
细胞标记和注射胚胎发展小鼠心脏
DOI:
10.3791/51356-v
•
07:20 min
•
April 17, 2014
•
Emilye Hiriart*
1
,
Patrick van Vliet*
2
,
Ralf J. Dirschinger
,
Sylvia M. Evans
,
Michel Puceat
1
INSERM UMR-910
,
Aix-Marseille University
,
2
Skaggs School of Pharmacy and Pharmaceutical Sciences
,
University of California, San Diego
Chapters
00:05
Title
01:49
Collection of E9.5 and E10.5 Embryos for Ex vivo Injection
03:10
Ex vivo DNA or Cell Injection Under a Stereomicroscope
04:35
Results: Embryonic Heart Cell Labeling and Injection
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一系列的方法来注入染料,DNA载体,病毒和细胞,以监测来自于胚胎或多能干细胞内的小鼠胚胎在胚胎天(E)9.5和后期阶段内源性和移植细胞的两个细胞命运和表型的发展。
Tags
Cell Labeling
Cell Injection
Embryonic Mouse Heart
Cell Fate
Embryonic Environment
Cell Lineage Tracing
Microinjection
Fluorescent Dyes
Virus
ShRNA
Stem Cell-derived Progenitor Cells
Organ Development
Embryo Culture
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