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蛋白通过流体动力基因传递在小鼠体内瞬时表达
JoVE Journal
Biology
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JoVE Journal Biology
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
DOI:

12:54 min

May 05, 2014

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Chapters

  • 00:05Title
  • 01:23Weighing Mice and Preparing Syringes
  • 03:38Hydrodynamic DNA Delivery
  • 08:18Large-volume Tail Vein Injection using an Alternative Hand Position
  • 09:41Representative Analysis of Transgene Expression
  • 11:50Conclusion

Summary

Automatic Translation

由流体动力基因递送裸DNA的体内转染引入基因到最小的炎症反应的动物的组织中。产生基因产物的足够量,使得基因功能和调节以及蛋白质结构和功能可以被分析。

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