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Journal
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Chemistry
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热生物催化:等温滴定量热法表征酶促反应
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Chemistry
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Please note that all translations are automatically generated.
Click here for the English version.
热生物催化:等温滴定量热法表征酶促反应
DOI:
10.3791/51487-v
•
13:00 min
•
April 04, 2014
•
Luca Mazzei
,
Stefano Ciurli
,
Barbara Zambelli
1
Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology
,
University of Bologna
Chapters
00:05
Title
02:01
Preparing Samples and Performing the Experiment
06:47
Data Analysis
09:24
Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry
12:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
等温滴定量热法测量热流释放或吸收的化学反应。该方法可用于定量酶催化。在本文中,该协议有助于建立,运行试验和数据分析通常被描述,并应用于酶水解尿素由刀豆脲酶的特性。
Tags
Isothermal Titration Calorimetry
ITC
Enzymatic Reactions
Enzyme Kinetics
Thermodynamics
Kcat
KM
Enthalpy
Urease
Jack Bean
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