Una enzima de restricción método basado clonación para evaluar la<em> In vitro</em> Capacidad de replicación del VIH-1 subtipo C Gag-MJ4 quiméricos virus
HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.