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研究DNA环接了单分子荧光共振能量转移
JoVE Journal
Biology
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JoVE Journal Biology
Studying DNA Looping by Single-Molecule FRET
DOI:

11:27 min

June 28, 2014

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Chapters

  • 00:05Title
  • 01:21Double Stranded (ds)DNA Sample Preparation
  • 02:52Flow Cell Preparation
  • 05:04Single-molecule Imaging
  • 06:18Image Processing and Data Analysis
  • 07:48Determining the J Factor
  • 09:44Results: Representative Analysis of DNA Looping
  • 10:52Conclusion

Summary

Automatic Translation

这项研究提出了一个详细的实验步骤,使用单分子荧光共振能量转移(FRET)来衡量的双链DNA循环动力学。该协议还描述了如何提取称为歼因子循环概率密度。

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