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Real-time Imaging af endotelcellefremkaldt celle vejkryds Under Neutrofil Transmigration under fysiologiske Flow
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Real-time Imaging af endotelcellefremkaldt celle vejkryds Under Neutrofil Transmigration under fysiologiske Flow

DOI:
10.3791/51766-v

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11:26 min

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August 14, 2014

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, , ,
1Department of Molecular Cell Biology,Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam

Chapters

  • 00:05Title
  • 01:59Plating and Maintenance of Human Umbilical Vein Endothelial Cells
  • 04:00Polymorphonuclear Leukocyte Isolation Using Percoll Gradients
  • 06:36Labeling of Endothelial Junctional VE-cadherin and PECAM-1 and Performing the PMN TEM Assay Under Flow
  • 09:24Results: Visualization of the Dynamics of VE-cadherin and PECAM-1 During Neutrophil TEM
  • 10:59Conclusion

Summary

Automatic Translation

Automatic Translation

Leukocytter krydser endotelmonolaget hjælp den paracellulære eller transcellulær vej. Vi udviklede en simpel analyse at følge fordelingen af ​​endogent forbindelsesepitop VE-cadherin og PECAM-1 under leukocyt transendotel migration under fysiologiske flow til at skelne mellem de to sjælevandring ruter.

Tags

Endothelial Cell-cell JunctionsNeutrophil TransmigrationPhysiological FlowLeukocyte Transendothelial MigrationParacellular RouteTranscellular RouteVE-cadherinPECAM-1In Vitro AssayFluorescent AntibodiesElectrical Cell-substrate Impedance SensingFRAP Measurements

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