JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在
小鼠睾丸
活体
显微注射和电穿孔
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
In Vivo
Microinjection and Electroporation of Mouse Testis
Please note that all translations are automatically generated.
Click here for the English version.
在
小鼠睾丸
活体
显微注射和电穿孔
DOI:
10.3791/51802-v
•
08:39 min
•
August 23, 2014
•
Marten Michaelis
,
Alexander Sobczak
,
Joachim M. Weitzel
1
Institute of Reproductive Biology
,
Leibniz Institute for Farm Animal Biology (FBN)
Chapters
00:05
Title
02:11
Anesthesia and Surgery
03:41
Microinjection and DNA Application
04:51
Electroporation of Testis
05:32
Wound Closure and Post-surgery
06:41
Results: In Vivo Microinjection and Electroporation of Mouse Testis
07:28
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了显微注射和小鼠睾丸中电
在体内
的睾丸小鼠细胞转染技术,研究精子的独特工艺。所提出的方案涉及以下步骤:玻璃毛细管制备,经由输出管显微注射,并通过电穿孔转染。
Tags
In Vivo Microinjection
Electroporation
Mouse Testis
Transfection
Spermatogenesis
Reporter Genes
Gene Transfer
Gene Function Analysis
DNA Constructs
RNA Constructs
Gene Regulation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
E15小鼠胚胎在子宫内侧脑室注射和电穿孔
在体内的单细胞电穿孔内完整的大脑发育
在子宫内
和
前体内的
基因表达在小鼠视网膜神经节细胞的电穿孔
使用哺乳动物骨骼肌的DNA转染
在体内
电穿孔
活体
成像和治疗卵巢癌的原位小鼠模型
激活细胞凋亡的细胞色素的细胞质显微注射
C</em
小鼠卵母细胞显微注射,成熟和倍性评估
生殖细胞的移植和睾丸组织在小鼠异种移植
在体内
电穿孔Morpholinos到成年斑马鱼视网膜
再生的成年斑马鱼尾鳍
在
吗啉代入
体内
电穿孔
Read Article