Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Krzysztof Bobik; John R. Dunlap; Tessa M. Burch-Smith

doi:10.3791/51844

Journal

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Biology

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جنبا إلى جنب تجميد الضغط العالي والسريع تجميد استبدال الأنسجة النباتية للنقل المجهر الإلكتروني

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

JoVE Journal
Biology

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JoVE Journal Biology

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

جنبا إلى جنب تجميد الضغط العالي والسريع تجميد استبدال الأنسجة النباتية للنقل المجهر الإلكتروني

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16,414 Views

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12:52 min

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October 13, 2014

DOI:

10.3791/51844-v

DOI:

10.3791/51844-v

•

12:52 min

October 13, 2014

•

16393 Views

Krzysztof Bobik¹, John R. Dunlap², Tessa M. Burch-Smith¹

¹Department of Biochemical, Cellular and Molecular Biology,University of Tennessee, Knoxville ²Advanced Microscopy and Imaging Facility,University of Tennessee, Knoxville

Summary

Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.