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12:52 min
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October 13, 2014
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Summary
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Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.
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Cite this Article
Bobik, K., Dunlap, J. R., Burch-Smith, T. M. Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy. J. Vis. Exp. (92), e51844, doi:10.3791/51844 (2014).
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