Journal
/
Biology
/
Detectie van alternatieve splicing Tijdens-mesenchymale transitie
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Please note that all translations are automatically generated. Click here for the English version.

Detectie van alternatieve splicing Tijdens-mesenchymale transitie

Nederlands

Automatically Generated

12,815 Views

•

11:48 min

•

October 09, 2014

DOI:

10.3791/51845-v

DOI:
10.3791/51845-v

•

11:48 min
October 09, 2014

•

6 Views
, ,
1Division of Hematology/Oncology, Department of Medicine, Robert H. Lurie Comprehensive Cancer Center,Northwestern University Feinberg School of Medicine

Summary

Automatically generated

Alternative splicing regulation has been shown to contribute to the epithelial-mesenchymal transition (EMT), an essential cellular program in various physiological and pathological processes. Here we describe a method utilizing an inducible EMT model for the detection of alternative splicing during EMT.

Explore More Videos

Alternative SplicingEpithelial-mesenchymal TransitionEMTTwistE-cadherinN-cadherinVimentinIsoform-specific PrimersQuantitative RT-PCRImmunoblotting

Read Article

Cite this Article

Huang, H., Xu, Y., Cheng, C. Detection of Alternative Splicing During Epithelial-Mesenchymal Transition. J. Vis. Exp. (92), e51845, doi:10.3791/51845 (2014).

Download .ris file

Copy

Share Video

.

Copy