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探讨高密度功能蛋白微阵列来检测蛋白质 - 蛋白质相互作用
JoVE Journal
Biology
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JoVE Journal Biology
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
DOI:

08:07 min

August 02, 2015

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Chapters

  • 00:05Title
  • 02:06Probe Preparation
  • 05:03Probing the Arrays
  • 06:57Results: Microarray Studies Reveal a Protein-protein Interactions Between Tda-V5 and Rim11
  • 07:41Conclusion

Summary

Automatic Translation

使用含有几乎整个S.蛋白质芯片酵母蛋白质组被探查以十万计的蛋白质-蛋白质相互作用中并行快速无偏询问。这种方法可以用于蛋白质 - 小分子,翻译后修饰,并在高通量其它测定。

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