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Sondering High-density funktionelt protein Microarrays at Detect protein-protein interaktioner
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JoVE Journal Biology
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Sondering High-density funktionelt protein Microarrays at Detect protein-protein interaktioner

DOI:
10.3791/51872-v

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08:07 min

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August 02, 2015

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, ,
1Department of Genetics,Stanford University, 2Stanford Center for Genomics and Personalized Medicine,Stanford University

Chapters

  • 00:05Title
  • 02:06Probe Preparation
  • 05:03Probing the Arrays
  • 06:57Results: Microarray Studies Reveal a Protein-protein Interactions Between Tda-V5 and Rim11
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

Anvendelse af protein microarrays indeholder næsten hele S. cerevisiae proteom probes til hurtig unbiased forhør af tusindvis af protein-protein interaktioner i parallel. Denne metode kan anvendes til protein-lille molekyle, posttranslationel modifikation og andre assays i high-throughput.

Tags

Keywords: Protein MicroarrayProtein-protein InteractionsKinaseYeastAffinity PurificationV5-epitope TagRecombinant ProteinsHigh-density ArrayInformatics AnalysisIn Vivo Validation

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