JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
膜蛋白的实时测量:受体相互作用使用表面等离子体共振(SPR)
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
Please note that all translations are automatically generated.
Click here for the English version.
膜蛋白的实时测量:受体相互作用使用表面等离子体共振(SPR)
DOI:
10.3791/51937-v
•
09:35 min
•
November 29, 2014
•
Nurit Livnat Levanon
,
Elena Vigonsky
,
Oded Lewinson
1
Department of Biochemistry, The Bruce and Ruth Rappaport Faculty of Medicine
,
The Technion-Israel Institute of Technology
Chapters
00:05
Title
01:25
Protein Sample and Buffer Preparation
02:38
Sensor Chip and SPR Instrument Setup
03:58
Experiment Cycles
07:32
Results: On Line Monitoring of Ligand Immobilization and Determination of Kinetic Rate Constants by Injections of Multiple Analyte Concentrations
09:07
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
表面等离子共振(SPR)是一种无标记检测方法,实时生物分子相互作用。在这里,协议的膜蛋白:受体相互作用实验说明,在讨论该技术的优点和缺点。
Tags
Surface Plasmon Resonance
SPR
Protein-protein Interactions
Membrane Proteins
Real-time Measurements
Label-free
Kinetics
Association
Dissociation
Sensogram
ABC Transporter
Substrate Binding Protein
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
实时定量PCR使用的Thermo Scientific的Solaris定量PCR检测
分析的预微RNA和小分子RNA采用实时定量PCR(定量PCR)阵列
确定基因表达概况
C。线虫
使用微阵列和实时PCR
实时监控的配体 - 受体相互作用的荧光共振能量转移
基于亲和力,外的相互作用筛选(AVEXIS)外低亲和力受体 - 配体相互作用的可扩展检测
视黄醇运输的血浆视黄醇结合蛋白的膜受体的实时分析
研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移
具有可变角度荧光显微镜植物细胞表面动力学的实时成像
扫描电子显微镜(SEM)协议有问题的工厂,卵菌和真菌样本
活细菌中蛋白质-蛋白质相互作用的FLIM-FRET测量。
Read Article