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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
A transfecção altamente eficiente de THP-1 por macrófagos humanos Nucleofection
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A transfecção altamente eficiente de THP-1 por macrófagos humanos Nucleofection

Article DOI: 10.3791/51960
September 2nd, 2014 •
1, 1, 1
1Institute of Nutrition, Friedrich Schiller University Jena

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September 2nd, 2014

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Click here for the English version.

Este protocolo apresenta um método eficaz e fiável para transfectar humanos THP-1, macrófagos com siRNA ou de ADN de plasmídeo por electroporação com alta eficiência de transfecção elevada, mantendo a vitalidade celular e capacidade total de diferenciação de macrófagos e de polarização.

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