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Immunology and Infection

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人THP-1巨噬细胞通过核转高效转染

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Please note that all translations are automatically generated. Click here for the English version.

人THP-1巨噬细胞通过核转高效转染

DOI:

10.3791/51960-v

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07:54 min

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September 02, 2014

•

Marten B. Maeß, Berith Wittig, Stefan Lorkowski

¹Institute of Nutrition,Friedrich Schiller University Jena

Chapters

00:05Title
01:20Predifferentiation of THP-1 Macrophage and Preparation for Nucleofection
03:22Nucleofection of THP-1 Macrophage
04:53Post-Nucleofection Care
06:15Gross Characterization of siRNA Transfection THP-1 Macrophages
07:21Conclusion

Summary

Automatic Translation

这个协议提出通过电穿孔转染人THP-1巨噬细胞中的siRNA或质粒DNA具有高转染效率，同时保持高的细胞活力和分化和极化全细胞巨噬细胞的能力的有效和可靠的方法。

Tags

Transfection Human THP-1 Macrophages Nucleofection SiRNA Plasmid DNA Innate Immune Response Cell Vitality Cell Behavior Cell Differentiation Cell Polarization Gene Regulation

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