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Von Fast Fluoreszenz-Imaging, um molekulare Diffusion Gesetz über Live Cell Membrane in einem kommerziellen Mikroskop
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Chapters
- 00:05Title
- 02:08System Calibration: Point Spread Function (PSF) Calibration
- 05:33Labeled Cell Preparation
- 08:18Data Acquisition Setup
- 10:06Imaging
- 12:39Representative Imaging Results
- 14:39Conclusion
Spatial distribution and temporal dynamics of plasma membrane proteins and lipids is a hot topic in biology. Here this issue is addressed by a spatio-temporal image fluctuation analysis that provides conceptually the same physical quantities of single particle tracking, but it uses small molecular labels and standard microscopy setups.
Tags
Fast Fluorescence ImagingMolecular DiffusionLive Cell MembranesCommercial MicroscopeSpatiotemporal ResolutionFluorescently-labeled Plasma-membrane Proteins And LipidsSpatial Autocorrelation FunctionMean Square DisplacementApparent DiffusivityTransferrin ReceptorATTO488 Labeled 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine
