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小鼠骨髓破骨细胞的推导
JoVE Journal
Biology
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JoVE Journal
Biology
Osteoclast Derivation from Mouse Bone Marrow
Please note that all translations are automatically generated.
Click here for the English version.
小鼠骨髓破骨细胞的推导
DOI:
10.3791/52056-v
•
06:17 min
•
November 06, 2014
•
Ruth Tevlin*
1
,
Adrian McArdle*
1,2
,
Charles K.F. Chan
,
John Pluvinage
,
Graham G. Walmsley
2
,
Taylor Wearda
2
,
Owen Marecic
2
,
Michael S. Hu
,
Kevin J. Paik
,
Kshemendra Senarath-Yapa
,
David A. Atashroo
,
Elizabeth R. Zielins
,
Derrick C. Wan
,
Irving L. Weissman
2
,
Michael T. Longaker
2
1
Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery
,
Stanford University School of Medicine
,
2
Institute for Stem Cell Biology and Regenerative Medicine
,
Stanford University
Chapters
00:05
Title
01:28
Media Preparation and Bone Marrow Isolation
03:05
Gradient Separation of Bone Marrow Cells and Cell Culturing
04:57
Results: Culturing Osteoclasts from Mouse Bone Marrow
05:56
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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italiano (Italian)
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Automatic Translation
破骨细胞是体内的主要骨吸收细胞。一个分离的破骨细胞中大量的能力已经导致破骨细胞生物学的理解显著进步。在这个协议中,我们描述了一种用于分离,培养和定量破骨细胞
的体外
活性。
Tags
Osteoclast
Bone Marrow
Monocyte/macrophage Lineage
Bone Resorption
Bone Remodeling
Osteoblasts
Skeletal Remodeling
Bone Matrix
Bone Formation
Bone Disease
Osteoporosis
In Vitro Osteoclast Isolation
Bone Remodeling Cycle
Therapeutic Strategies
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