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Visualiseren Non-lytische Exocytosis van<em> Cryptococcus neoformans</em> Van Macrofagen behulp Digital Light Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy
DOI:

10:02 min

October 21, 2014

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Chapters

  • 00:05Title
  • 01:27Isolation and Preparation of Primary Bone Macrophages from C57/B16 Mice
  • 04:51Co‐incubation of Primary Murine Macrophages and C. neoformans
  • 06:56Visualizing Non‐lytic Exocytosis in Real‐time Utilizing Digital Light Microscopy
  • 08:11Results: Primary Murine Macrophages Undergo Different Types of Non‐lytic Exocytosis of C. neoformans
  • 09:36Conclusion

Summary

Automatic Translation

We beschrijven hoe om te visualiseren macrophage- C. neoformans (Cn) interacties in real time, met bijzondere nadruk op het proces van niet-lytische exocytose middels digitale lichtmicroscopie. Met deze techniek individueel geïnfecteerde macrofagen kunnen worden bestudeerd om verschillende aspecten van dit verschijnsel bepalen.

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