/

/

在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Please note that all translations are automatically generated. Click here for the English version.

在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

DOI:

10.3791/52104-v

•

12:44 min

•

November 11, 2014

•

Shan Zong*¹, Shuyun Deng*¹, Kenian Chen, Jia Qian Wu

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center,The University of Texas Graduate School of Biomedical Sciences at Houston

Chapters

00:05Title
02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
03:25EML Cell Culture and Depletion of Lineage Positive Cells
04:52Separation of Lin–CD34⁺ and Lin–CD34^– Cells
07:15RNA Preparation and Library Construction for High-throughput Sequencing
09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
11:38Conclusion

Summary

Automatic Translation

RNA测序和生物信息学分析被用来确定显著和差异表达的转录因子在小鼠EMLcells林-CD34 +和林-CD34-细胞亚群。这些转录因子可能在确定自我更新林的CD34 +和偏微分林CD34-细胞间的开关发挥重要作用。

Tags

RNA-sequencing EML Cells Hematopoietic Stem Cells Self-renewal Differentiation Transcription Factors Gene Expression Lin-CD34+ Cells Lin-CD34- Cells RNA Isolation Library Construction Data Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从胎盘中的造血干细胞的分离和分析

SC1（Pluripotin）使用，以支持在没有LIF的自我更新MESC

表型分析和分离小鼠造血干细胞和传承的承诺祖

Oct4GiP记者试验研究小鼠胚胎干细胞维持和自我更新的基因调节

监控功能和血管形态由快速增长的肿瘤产生细胞分泌的因素招募

在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

小鼠胚胎的逆转录病毒感染干细胞衍生胚体细胞造血分化的分析

糖皮质激素粪分析：在马属动物无创监测肾上腺

通过光谱流式细胞仪由固态组织中分离出悬浮细胞系的分析

在体内人细胞 Rb 蛋白 sumo 的检测与分析

Read Article