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在EML造血前体细胞调节自我更新和分化的关键因素,通过RNA测序分析鉴定
JoVE Journal
Biology
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JoVE Journal Biology
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
DOI:

12:44 min

November 11, 2014

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Chapters

  • 00:05Title
  • 02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
  • 03:25EML Cell Culture and Depletion of Lineage Positive Cells
  • 04:52Separation of Lin–CD34+ and Lin–CD34 Cells
  • 07:15RNA Preparation and Library Construction for High-throughput Sequencing
  • 09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
  • 11:38Conclusion

Summary

Automatic Translation

RNA测序和生物信息学分析被用来确定显著和差异表达的转录因子在小鼠EMLcells林-CD34 +和林-CD34-细胞亚群。这些转录因子可能在确定自我更新林的CD34 +和偏微分林CD34-细胞间的开关发挥重要作用。

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