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Identificatie van Key factoren die de Self-vernieuwing en differentiatie in EML Hematopoietic voorloper cellen door RNA-sequencing Analyse
JoVE Journal
Biology
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JoVE Journal Biology
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
DOI:

12:44 min

November 11, 2014

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Chapters

  • 00:05Title
  • 02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
  • 03:25EML Cell Culture and Depletion of Lineage Positive Cells
  • 04:52Separation of Lin–CD34+ and Lin–CD34 Cells
  • 07:15RNA Preparation and Library Construction for High-throughput Sequencing
  • 09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
  • 11:38Conclusion

Summary

Automatic Translation

RNA-sequencing en bioinformatica analyses werden significant verschillend tot expressie en transcriptiefactoren in Lin-CD34 + en CD34-Lin subpopulaties van muis EMLcells identificeren. Deze transcriptiefactoren kunnen een belangrijke rol spelen bij het bepalen van de switch tussen zelfvernieuwende Lin-CD34 + en gedeeltelijk gedifferentieerd Lin-CD34-cellen.

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