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1Apoptosis Research Centre, Children’s Hospital of Eastern Ontario Research Institute and Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 2Montreal Neurological Institute, 3Apoptosis Research Centre, Children’s Hospital of Eastern Ontario Research Institute and Department of Pediatrics,University of Ottawa
Chapters
- 00:05Title
- 01:25Cell Lysis and Ultracentrifugation
- 04:06Gradient Fractionation System Instrument Set-up
- 06:48Gradient Fractionation and Collection of Samples
- 08:12Results: Polysome Profiling Identifies PDCD4 as a Selective Inhibitor of XIAP and Bcl-xL Translation
- 09:37Conclusion
The ability of cells to adapt to stress is crucial for their survival. Regulation of mRNA translation is one such adaptation strategy, providing for rapid regulation of the proteome. Here, we provide a standardized polysome profiling protocol to identify specific mRNAs that are selectively translated under stress conditions.
