Cosa fare e cosa non fare di Cryo-microscopia elettronica: A Primer su preparazione del campione e raccolta dati di alta qualità per la ricostruzione macromolecolare 3D
This paper describes the cryo-electron microscopy methodology, which is used to obtain high quality microscopic images of macromolecules in their near-native state. The method yields images suitable for further computerized processing using the single particle approach, devised to generate the 3D reconstruction of a macromolecule.