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マウスにおける安定な高レベルの骨髄キメラ現象を生成するための骨髄抑制剤としてブスルファン
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JoVE Journal Medicine
Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice
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マウスにおける安定な高レベルの骨髄キメラ現象を生成するための骨髄抑制剤としてブスルファン

日本語

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13,029 Views

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11:25 min

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April 01, 2015

DOI:

10.3791/52553-v

DOI:
10.3791/52553-v

•

11:25 min
April 01, 2015

•

6 Views
, , , , ,
1Department of Biomedical Physiology and Kinesiology,Simon Fraser University, 2The Biomedical Research Centre,University of British Columbia, 3Division of Neurology, Department of Medicine, Neuromuscular Disease Unit,VHHSC

Summary

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We describe a protocol whereby busulfan conditioning permits the bone marrow of a recipient mouse to be replaced with bone marrow cells from donor mice ubiquitously expressing green fluorescent protein, in the absence of irradiation. This technique is useful to study bone marrow cell accumulation in the central nervous system.

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Bone Marrow TransplantationMyeloablative ConditioningBusulfanChimerismGreen Fluorescent ProteinCentral Nervous SystemNeurodegenerationGenetic Engineering

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Cite this Article

Peake, K., Manning, J., Lewis, C., Barr, C., Rossi, F., Krieger, C. Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice. J. Vis. Exp. (98), e52553, doi:10.3791/52553 (2015).

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