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建立一个人类多发性骨髓瘤异种移植模型中的鸡，研究肿瘤的生长，侵袭和血管生成

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Medicine

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JoVE Journal Medicine

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

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建立一个人类多发性骨髓瘤异种移植模型中的鸡，研究肿瘤的生长，侵袭和血管生成

DOI:

10.3791/52665-v

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10:04 min

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May 01, 2015

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Agnieszka Martowicz*^1,4, Johann Kern*^1,2, Eberhard Gunsilius, Gerold Untergasser³

¹Department of Internal Medicine V,Innsbruck Medical University, ²Oncotyrol GmbH, ³Tyrolean Cancer Research Institute, ⁴Division of Vascular Biology, Department of Medical Biochemistry and Biophysics,Karolinska Institute

Chapters

00:05Title
02:09Cell Culture and Lentiviral Transfection
03:203D-Multiple Myeloma Spheroid Model
04:473D Multiple Myeloma Xenograft Model in the CAM
06:17Quantification of eGFP Protein by ELISA
07:03Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells
08:05Results: Bortezomib Treatment of Multiple Meyloma Xenografts
09:17Conclusion

Summary

Automatic Translation

人多发性骨髓瘤（MM）细胞需要间质细胞和细胞外基质成分为存活和增殖的支持性微环境。我们建立了与植入人类骨髓瘤和间质细胞体内鸡胚模型来研究癌症药物对肿瘤的生长，侵袭和血管生成作用。

Tags

Keywords: Multiple Myeloma Xenograft Model Chicken Chorioallantoic Membrane 3D Culture Angiogenesis Tumor Growth Invasion GFP Reporter Mesenchymal Cells Collagen RPMI-8226 OPM-2 Therapeutic Screening

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