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Imágenes de células vivas durante el estiramiento mecánico
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Live Cell Imaging during Mechanical Stretch
DOI:

07:42 min

August 19, 2015

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Chapters

  • 00:05Title
  • 01:29Application of Stretch on Mouse Lung Epithelial Cell Line
  • 03:22Application of Stretch and Atomic Force Microscopy
  • 05:15Nano-indentation of Stretched Cells
  • 05:44Results: Elastic Modulus Mapping of Mouse Lung Epithelial Cells Under Strain
  • 06:57Conclusion

Summary

Automatic Translation

A novel imaging protocol was developed using a custom motor-driven mechanical actuator to allow the measurement of real time responses to mechanical strain in live cells. Relevant to mechanobiology, the system can apply strains up to 20% while allowing near real-time imaging with confocal or atomic force microscopy.

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