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Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

DOI:
10.3791/52753-v

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09:07 min

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May 27, 2015

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, , , , ,
1Department of Pathology,Stanford University School of Medicine, 2Department of Microbiology & Immunology,Stanford University School of Medicine

Chapters

  • 00:05Title
  • 01:59Generation and Characterization of Bone Marrow-derived Cultured Mast Cells (BMCMCs)
  • 03:48Engraftment of Mast Cell-deficient Mice with BMCMCs
  • 05:30Peritoneal Mast Cell Engraftment Analysis
  • 06:50Results: Representative BMCMC In Vitro Culture and In Vivo Engraftment
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.

Tags

Keywords: Mast CellsIn VivoKnock-in MiceBone Marrow-derived Cultured Mast CellsAdoptive TransferAnatomical DistributionHost DefenseAllergic Reactions

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