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HeLa Basert Cell ytringsfrihet Systemer for Expression of<em> Plasmodium</em> Rhoptry Proteiner
JoVE Journal
Biology
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JoVE Journal Biology
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
DOI:

09:03 min

June 10, 2015

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Chapters

  • 00:05Title
  • 02:12Protein Expression Using In Vitro Human Cell Free Expression System
  • 03:21Purification of Expressed Recombinant Proteins
  • 05:05Western Blotting
  • 06:52Results: Purple Color Band on Nitrocellulose Paper During Developing Step Confirms the Successful Expression of Proteins
  • 08:11Conclusion

Summary

Automatic Translation

Uttrykk for malaria proteiner i cellebaserte systemer er fortsatt utfordrende. Vi viser to trinn og ett skritt IVT (in vitro oversettelse) celle gratis ekspresjonssystemer for å uttrykke malaria rekombinante rhoptry proteiner fra HeLa-celler. Vi bruker et Ni-resin affinitet baserte rensesystem for å rense rhoptry proteiner.

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