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흉선의 특성 사용하여 마우스 배아에서 전구 세포를 정착<em> 생체</em>와<em> 체외</em> 분석 실험
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
DOI:

08:56 min

June 09, 2015

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Chapters

  • 00:05Title
  • 01:51Embryo Dissection
  • 02:52Thymus Isolation
  • 03:49E14 Thymic Lobe Progenitor Colonization: Hanging Drop Technique
  • 04:53Grafting Under the Kidney Capsule
  • 06:22Results: Representative Thymic Settling Progenitor (TSP) Propagation
  • 08:07Conclusion

Summary

Automatic Translation

This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

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