06:30Ratiometric Confocal Fluorescence Measurements of Intracellular Calcium Concentration in the Podocytes
08:08Image Analysis for the Calcium Measurements
09:46Results: Recording of Single Ion Channels Activity in the Podocytes of Freshly Isolated Glomeruli and Real-time Detection of Calcium Concentration Changes
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.