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Neuroscience
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海马神经元活动的可视化免疫组化空间后学习神经发育障碍的小鼠模型
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
Please note that all translations are automatically generated.
Click here for the English version.
海马神经元活动的可视化免疫组化空间后学习神经发育障碍的小鼠模型
DOI:
10.3791/52919-v
•
07:43 min
•
May 12, 2015
•
Giovanni Provenzano
,
Luca Pangrazzi
,
Andrea Poli
,
Nicoletta Berardi
,
Yuri Bozzi
2
1
Centre for Integrative Biology (CIBIO)
,
University of Trento
,
2
CNR Neuroscience Institute, Pisa, Italy
Chapters
00:05
Title
01:30
Preparation of Brain Sections
02:16
pERK Immunohistochemistry
05:22
Bright-field Imaging of Neuron Activation
05:43
Results: pERK is a Reliable Molecular Readout of Learning Dependent Neuronal Activation
06:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个协议,免疫组织化学研究海马神经元激活的轮廓暴露于空间学习任务为特征的神经发育起源的认知缺陷小鼠模型。此协议可应用到特征的认知缺陷都遗传或药理学小鼠模型。
Tags
Immunohistochemistry
PERK
Hippocampus
Spatial Learning
Morris Water Maze
Engrailed-2 Knockout
Autism Spectrum Disorders
Neurodevelopmental Disorders
Neuronal Activation
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