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Visualisering af Chondrocyt Intercalation og Directional spredning via Zebrabow Klonal Cell Analysis i Embryonale Meckel s Brusk
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
DOI:

06:40 min

October 21, 2015

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Chapters

  • 00:05Title
  • 01:21Embryo Collection, Tamoxifen Treatment, and Embryo Selection
  • 02:46Mounting Embryos in Methylcellulose and Fluorescence Microscopy
  • 04:33Results: Chondrocyte Intercalation and Directional Proliferation in ubi:Zebrabow-M;sox10:ERT2-Cre Transgenic Zebrafish
  • 06:15Conclusion

Summary

Automatic Translation

Celle organisering af kraniofaciale knogler har længe været en hypotese, men aldrig direkte visualiseres. Multispektral cellemærkning og in vivo levende billeddannelse tillader visualisering af dynamiske celle adfærd i zebrafisk underkæbe. Her har vi detaljeret protokollen til at manipulere Zebrabow transgene fisk og direkte observere celle intercalation og morfologiske ændringer af chondrocytter i Meckel s brusk.

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