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Adaptação do Processo Electrospinning para fornecer três ambientes únicos para um Tri-camadas<em> In Vitro</em> Modelo da parede das vias aéreas
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1Division of Drug Delivery and Tissue Engineering,University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy,University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences,University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences,University of Nottingham, 5NIHR Respiratory Biomedical Research Unit,University of Leicester, 6School of Sport, Exercise, and Health Sciences,Loughborough University
Chapters
- 00:05Title
- 01:49Electrospinning Biphasic and Aligned Polyethylene Terephthalate Scaffolds
- 05:34Sterilization of Scaffolds
- 06:24Seeding of Fibroblasts and Epithelial Cells onto Biphasic Scaffolds and Airway Smooth Muscle onto Aligned Scaffolds
- 08:36Tri-culture Using a Bioreactor System
- 09:29Results: Tri-Culture Cells Continue to Produce Cell Type Specific Proteins
- 10:20Conclusion
Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.
