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Una célula de ensayo libre para el Estudio de la cromatina descondensación en la final de la mitosis
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JoVE Journal Biology
A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis
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Una célula de ensayo libre para el Estudio de la cromatina descondensación en la final de la mitosis

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10,157 Views

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11:04 min

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December 19, 2015

DOI:

10.3791/53407-v

DOI:
10.3791/53407-v

•

11:04 min
December 19, 2015

•

2 Views
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1Friedrich Miescher Laboratory,Max Planck Society, 2Nencki Institute of Experimental Biology,Polish Academy of Sciences

Summary

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The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.

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Schellhaus, A. K., Magalska, A., Schooley, A., Antonin, W. A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis. J. Vis. Exp. (106), e53407, doi:10.3791/53407 (2015).

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