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January 14, 2016
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مناعي لونين هي تقنية قوية لتحديد المواقع DNA من البروتينات في الجسم الحي نبات الأرابيدوبسيس ملزمة. ويشمل هذا الإجراء لونين عبر ربط والتشرذم، مناعي مع الأجسام المضادة انتقائية ضد البروتين من الفائدة، وتحليل QPCR من الحمض النووي ملزمة. نحن تصف مقايسة الشذرة بسيط الأمثل لمحطات نبات الأرابيدوبسيس.
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Cite this Article
Komar, D. N., Mouriz, A., Jarillo, J. A., Piñeiro, M. Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo. J. Vis. Exp. (107), e53422, doi:10.3791/53422 (2016).
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