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Chapters
- 00:05Title
- 01:11Crosslinking of Plant Material
- 02:53Preparation of Antibodies
- 04:26Chromatin Extraction
- 07:25Immunoprecipitation of the Protein of Interest and Rescue of DNA
- 09:45Results: Chromatin Immunoprecipitation Assay to Assess EBS-DNA Interactions in Arabidopsis
- 11:13Conclusion
Kromatin immunofældning er en kraftfuld teknik til identifikation af DNA-bindingssteder fra Arabidopsis proteiner in vivo. Denne procedure omfatter chromatin tværbinding og fragmentering, immunfældning med selektive antistoffer mod proteinet af interesse og qPCR analyse af bundne DNA. Vi beskriver en enkel chip assay optimeret til Arabidopsis planter.
