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JoVE Journal
Biology

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Chromatin Immunpræcipitation Assay til identifikation af Arabidopsis Protein-DNA interaktioner
 
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Chromatin Immunpræcipitation Assay til identifikation af Arabidopsis Protein-DNA interaktioner

Article DOI: 10.3791/53422
January 14th, 2016

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Summary January 14th, 2016

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Kromatin immunofældning er en kraftfuld teknik til identifikation af DNA-bindingssteder fra Arabidopsis proteiner in vivo. Denne procedure omfatter chromatin tværbinding og fragmentering, immunfældning med selektive antistoffer mod proteinet af interesse og qPCR analyse af bundne DNA. Vi beskriver en enkel chip assay optimeret til Arabidopsis planter.

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