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协议中再生的小鼠肠道使用条件潘氏细胞中的作用分析<em>的Cre-LOX</em>小鼠模型
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
DOI:

07:48 min

November 21, 2015

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Chapters

  • 00:05Title
  • 00:33Intestine Dissection
  • 01:32Formalin Fixation
  • 02:25Methacarn Fixation
  • 03:38Whole Mount LacZ Visualization
  • 06:01Results: Representative Intestinal Epithelial Cell Characterization After Gene Manipulation
  • 07:20Conclusion

Summary

Automatic Translation

肠上皮干细胞(ISCS)是夹杂着潘氏细胞。这些细胞分化的ISC,支持综合监控系统,并提供抗菌保护后代。在这里,我们将演示如何使用条件转基因小鼠模型,建立了潘氏细胞在维持肠道上皮细胞至关重要的作用。

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