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体内生物传感器跟踪非凋亡胱天蛋白酶的活动在果蝇
JoVE Journal
Biology
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JoVE Journal Biology
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
DOI:

13:21 min

November 27, 2016

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Chapters

  • 00:05Title
  • 01:48Preparing the CaspaseTracker Flies and Dissected Tissues
  • 04:30Tissue Staining and Mounting
  • 08:11Confocal Microscopy
  • 09:37Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
  • 12:10Conclusion

Summary

Automatic Translation

以检测健康细胞在包含蛋白酶的活性水平低的整体动物, 果蝇生成指定CaspaseTracker高度敏感的生物传感器。依赖性的caspase-生物传感器的活性在长寿命的健康细胞中检测遍布在没有死亡刺激的最佳条件下饲养成年动物的内部器官。

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